Year 2018, Volume 3, Issue 4, Pages 206 - 214 2018-12-29

The Clinical Importance of Hyponatremia in Pediatric Intensive Care Unit
Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi

Fatih AYGÜN [1] , Seda ARAS [2] , Fatih VAROL [3] , Ahmet İRDEM [4]

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Circulating tumor cells (CTCs) play a crucial role in the metastatic spread of carcinoma. Therefore, CTC has been interest of a subject in the past few decades in terms of prognosis and response to the therapy in several cancer diseases. Recent improvements in technical approaches maintain to identify CTCs from whole blood have demonstrated the potential value of CTC detection as a liquid biopsy especially in those tumors where tissue accessibility is often challenging as in lung cancer. Lung cancer is the most common cause of death from cancer worldwide in both men and women which is commonly metastasize before it is diagnosed. The aim of this study is to enumerate of CTCs in peripheral blood sample (7.5 mL) of lung cancer patients by flow cytometry.  Our modified method which consists of enrichment and detection steps get involved in 9 patients with lung cancer and 9 healthy volunteers. We performed a density-based ficoll gradient centrifugation and a immunomagnetic separation technique (CD45 negative selection) for the enrichment step.  Next, multi-parameter flow cytometry based on the expression of anti-epithelial cell adhesion molecule and cytokeratins was used to detect circulating tumor cells among enriched cells. According to our results,  circulating tumor cells were not detected on healthy volunteers but circulating tumor cells were found in all of patients with lung cancer (Z=3.823; p<0.001). We demonstrate that circulating tumor cells were detectable in peripheral blood sample of lung cancer patients by our modified method.

Hiponatremi çocuk acil ve yoğun bakım ünitelerinde sık görülen bir elektrolit bozukluğudur. Yetişkin hastalarda hiponatreminin mortalite için bağımsız bir risk faktörü olduğu gösterilmesine karşın özellikle çocuk yoğun bakım (ÇYB) hastalarında hiponatreminin klinik önemi ve prognoza etkisi yeterince irdelenmemiştir. Bu çalışmada çocuk yoğun bakım ünitesi’nde (ÇYBÜ) yatırılan hastalarda ilk gün hiponatremi sıklığı ve erken dönem prognoz ile olan ilişkisinin araştırılması planlanmıştır. Ekim 2016 ile Mart 2018 tarihleri arasında yatırılan tüm hastaların verileri geriye dönük olarak incelenmiştir. Çalışmaya alınan 435 hastanın 22’sinde orta ve ağır düzeyde hiponatremi saptandı. Hastaların 12’si (%54,5) erkek, 10’u (%45,5) kızdı. Yaş dağılımları 1,5 ay ile 17 yaş arasında olup ortalama hasta yaşı 3,67±2,00 yıldı. Hiponatremi saptanan hastaların ortalama yatış süresi 14,91±22,17 gündü. Serum sodyum (Na) düzeyinin düşüklüğü (Na<130 mmol/L) ile mekanik ventilatöre bağlanma, inotrop ilaç kullanımı, ÇYBÜ yatış süresi, çocuk mortalite  (PRISM ) skoru, kan ürünü kullanımı, ölüm, akut böbrek hasarı ve kalp yetmezliği arasında istatistiksel anlamlı ilişki saptandı. Çocuk hastalarda ilk başvuru sırasında Na<130mml/L olması mortalite ve morbidite ile ilişkili önemli bir prognostik belirteç olarak değerlendirilmelidir.

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Primary Language tr
Subjects Health Care Sciences and Services
Journal Section Research article
Authors

Orcid: 0000-0001-6519-6583
Author: Fatih AYGÜN (Primary Author)
Institution: İstanbul Üniversitesi-Cerrahpaşa, Cerrahpaşa Tıp Fakültesi, Çocuk Yoğun Bakım Bilim Dalı
Country: Turkey


Orcid: 0000-0001-9000-9014
Author: Seda ARAS
Institution: Sağlık Bilimleri Üniversitesi, Sağlık Uygulama ve Araştırma Merkezi, Okmeydanı Eğitim Araştırma Hastanesi, Çocuk Sağlığı ve Hastalıkları

Orcid: 0000-0002-2424-6887
Author: Fatih VAROL
Institution: İstanbul Üniversitesi-Cerrahpaşa, Cerrahpaşa Tıp Fakültesi, Çocuk Yoğun Bakım Bilim Dalı
Country: Turkey


Orcid: 0000-0002-2565-5674
Author: Ahmet İRDEM
Institution: Sağlık Bilimleri Üniversitesi, Sağlık Uygulama ve Araştırma Merkezi, Okmeydanı Eğitim Araştırma Hastanesi, Çocuk Kardiyolojisi

Bibtex @research article { otjhs434105, journal = {Online Türk Sağlık Bilimleri Dergisi}, issn = {}, eissn = {2459-1467}, address = {Sakarya University}, year = {2018}, volume = {3}, pages = {206 - 214}, doi = {10.26453/otjhs.434105}, title = {Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi}, key = {cite}, author = {AYGÜN, Fatih and ARAS, Seda and VAROL, Fatih and İRDEM, Ahmet} }
APA AYGÜN, F , ARAS, S , VAROL, F , İRDEM, A . (2018). Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi. Online Türk Sağlık Bilimleri Dergisi, 3 (4), 206-214. DOI: 10.26453/otjhs.434105
MLA AYGÜN, F , ARAS, S , VAROL, F , İRDEM, A . "Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi". Online Türk Sağlık Bilimleri Dergisi 3 (2018): 206-214 <http://www.otjhs.sakarya.edu.tr/issue/39039/434105>
Chicago AYGÜN, F , ARAS, S , VAROL, F , İRDEM, A . "Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi". Online Türk Sağlık Bilimleri Dergisi 3 (2018): 206-214
RIS TY - JOUR T1 - Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi AU - Fatih AYGÜN , Seda ARAS , Fatih VAROL , Ahmet İRDEM Y1 - 2018 PY - 2018 N1 - doi: 10.26453/otjhs.434105 DO - 10.26453/otjhs.434105 T2 - Online Türk Sağlık Bilimleri Dergisi JF - Journal JO - JOR SP - 206 EP - 214 VL - 3 IS - 4 SN - -2459-1467 M3 - doi: 10.26453/otjhs.434105 UR - https://doi.org/10.26453/otjhs.434105 Y2 - 2018 ER -
EndNote %0 Online Turkish Journal of Health Sciences Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi %A Fatih AYGÜN , Seda ARAS , Fatih VAROL , Ahmet İRDEM %T Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi %D 2018 %J Online Türk Sağlık Bilimleri Dergisi %P -2459-1467 %V 3 %N 4 %R doi: 10.26453/otjhs.434105 %U 10.26453/otjhs.434105
ISNAD AYGÜN, Fatih , ARAS, Seda , VAROL, Fatih , İRDEM, Ahmet . "Çocuk Yoğun Bakım Hastalarında Hiponatreminin Klinik Önemi". Online Türk Sağlık Bilimleri Dergisi 3 / 4 (December 2018): 206-214. https://doi.org/10.26453/otjhs.434105